potential protective and therapeutic effects of aloe vera juice on malathion induced hepatotoxicity in rabbits

the potential protective and therapeutic effects of Aloe vera juice against malathion induced hepatotoxicity were evaluated in this study. one hundred twelve young male rabbits were used ; they were allocated into two sets of experiments included rabbits treated for short [7 days] and long [21 days] periods. Animals of the first set [short period of treatment] were divided into eight groups; each consisted of four treated groups and four control groups [each treated group had its own control]. The animals of the first group were orally dosed with Aloe vera juice [0.84 ml/kg b. wt]. Rabbits of the second group were orally dosed with malathion [5 mg/kg b. wt.]. The third group animals were dosed orally with malathion concomitant with Aloe vera juice [this group served as the protective group]. Animals of the fourth group were dosed orally with malathion; for 7 days followed by Aloe vera juice for the same period [this group served as the therapeutic group]. The design of the second set [long period of treatment] was exactly similar to that of the short period experiments [divided into eight groups; four treated and four control groups] except the duration of treatment which extended to 21 days. Animals of the control groups of Aloe vera treated rabbits were dosed with distilled water, and those of malathion treated animals were dosed with the solvent of the insecticide. All the animals were sacrificed, blood samples were collected and the serum was used to determine the levels of hepatic enzyme markers: lactate dehydrogenase [LDH], aspartate aminotransferase [ASAT], alanine aminotransferase [ALAT] and alkaline phosphatase [ALP] as well as the total bilirubin. the results revealed that treatment of rabbits with malathion caused marked increase in the serum activity of LDH, ASAT, ALAT and ALP in treated rabbits. Administration of Aloe vera juice [in the protective and therapeutic groups] was found to be effective in lowering the elevated activities of these enzymes to approximate near normal levels for both the short and long periods of treatment, especially in the activity of LDH, ASAT and ALP. The present results suggested that Aloe vera juice has ameliorative effects against hepatotoxicity produced by malathion in the treated rabbits

potential protective and therapeutic effects of aloe vera juice against malathion induced haematological changes in rabbits

The potential protective and therapeutic effects of Aloe vera juice against malathion induced haematological changes in young rabbits [Oryctolagus cuniculus] were evaluated in this study. Rabbits were allocated into two sets of experiments short [7 days] and long [21 days] periods. Animals of each set were divided into eight groups; four treated groups and each treated group had its own control. The animals of the first group were orally dosed with Aloe vera juice [0.84 ml/kg b. wt.]. Rabbits of the second group were orally dosed with malathion [5 mg/kg b. wt.]. In the third group [the protective group] animals were dosed orally with malathion concomitant with Aloe vera juice. Animals of the fourth group [the therapeutic group] were dosed orally with malathion; for 7 days followed by Aloe vera juice for the same period. All the animals were sacrificed, blood samples were collected and used to determine the levels of hematological indices: red blood cells [RBCs], white blood cells [WBCs], haemoglobin [Hb], haematocrit [Hct], mean corpuscular volume [MCV], mean corpuscular haemoglobin [MCH] and mean corpuscular haemoglobin concentration [MCHC]. Rabbits treated with Aloe vera juice [group 1] showed insignificant change in WBCs count, MCH and MCHC after treatment for both periods. On the other hand, other parameters exhibited significant [RBCs count] and highly significant [Hb and Hct values] change in rabbits treated for the short period. The rabbits of the second group [treated with malathion] exhibited a marked increase in WBCs count, MCV and MCH values, while, the values of RBCs, Haemoglobin and Hct were decreased. The protective group [Group3] showed that the values of all the tested haematological parameters [except MCHC, in both periods of treatment, and WBCs count in the short period of treatment] recorded a significant change in rabbits treated for short [7 days] or long [21 days] periods. A marked recovery in RBCs count and MCH value was observed in rabbits treated for 21 days. Regarding to group 4 [the therapeutic group], it was found that in both terms the rabbits exhibited insignificant changes in the values of all the tested haematological parameters compared to their corresponding controls, except three parameters in which they recorded significant [WBCs count, MCHC] and highly significant [Hct] increase compared to their control values after the long period only. These observations indicated the ability of Aloe vera juice to improve the deleterious effects caused by malathion administration